Ponceau S, Bioreagent for electrophoresis, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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M-Clarity™ quality level = MQ200

P3504-100G P3504-10G P3504-50G
P3504-100GEA 268 GBP
P3504-100G P3504-10G P3504-50G
Ponceau S, Bioreagent for electrophoresis, Sigma-Aldrich®
Ponceau S

Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection. The following are two common stain formulations: 0.1% Ponceau S (w/v) in 5% acetic acid 2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid.


  • Ponceau S has been used for membrane staining (Western blotting)

Formula: C₂₂H₁₂N₄Na₄O₁₃S₄
Storage Temperature: Room Temperature
MDL Number: MFCD00003892
CAS Number: 6226-79-5
EINECS: 228-319-2
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Specification Test Results

Infrared spectrum Conforms to Structure
Solubility (Turbidity) Clear
Solubility (Colour)
c = 4mg/4ml; Water
Red to Very Dark Red
Suitability
Suitable for Use as a Stain in Electrophoresis
Suitable
Wavelength
C = 0.015 G/L, Di water
518 - 522 nm
Extinction Coefficient ≥33000

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