FITC (fluorescein-5-isothiocyanate, fluorescein isothiocyanate isomer I) ≥97.5% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY
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M-Clarity™ quality level = MQ100

F4274-250MG F4274-50MG
F4274-250MGEA 503 GBP
F4274-250MG F4274-50MG
FITC (fluorescein-5-isothiocyanate, fluorescein isothiocyanate isomer I) ≥97.5% (by HPLC), Sigma-Aldrich®
FITC (Fluorescein 5-isothiocyanate, Fluorescein isothiocyanate isomer I)

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins. Fluorescein isothiocyanate isomer I has been used for the staining of conidia.
Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC).


Packaging: Bottomless glass bottle. Contents are inside inserted fused cone.

Formula: C₂₁H₁₁NO₅S
Melting Pt: >360 °C (lit.)
Storage Temperature: Refrigerator
MDL Number: MFCD00005063
CAS Number: 3326-32-7
EINECS: 222-042-0
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Specification Test Results

Appearance (colour) Yellow to very dark yellow and orange to very dark orange
Appearance (form) Powder
Purity (hplc area %) ≥97.5%
Solubility (colour) Yellow to very dark yellow and green-yellow to very dark green-yellow
Solubility (turbidity) Clear
Solubility (method) 10 mg/ml acetone
Infrared spectrum Conforms to structure
Recommended retest period 45 months

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