Blotting membranes, Nytran® SuPerCharge, Whatman™

Supplier: Whatman products (Cytiva)
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Whatman™ Nytran®
732-4066EA 653 GBP
732-4066 732-4065 732-0286
Blotting membranes, Nytran® SuPerCharge, Whatman™
Transfer Membranes
Nytran® SuPerCharge (SPC) nylon membranes have a very high positive charge and a higher density of nylon per unit area. Available as circles, sheets or rolls for microplates.

  • Consistently gives high signal with extremely low background
  • Membrane is cast uniformly on both sides of a support matrix, providing excellent symmetry and allowing the membrane to lie flat without curling
  • Uniform pore size and distribution, and a surface free of microvoids, allows greater reproducibility of results across a membrane and from blot to blot

Nytran SPC membranes show a very uniform pore size and pore distribution compared to typical nylon membranes. They are free of surface microvoids, which are common in other membranes. These characteristics lead to greater reproducibility of results across a membrane and from blot to blot. With typical manufacturing techniques, increasing positive charge tends to increase background. Nytran SPC membranes are manufactured using a process that allows the combination of high positive charge with low background. Whether using radioactive or nonradioactive detection techniques, Nytran SPC consistently gives high signal with extremely low background.

These membranes are designed for Southern and Northern blotting, colony and plaque lifts, and dot- or slot-blots. Membranes are cast uniformly for excellent symmetry and to prevent curling. Nytran SPC nylon membranes have a very high positive charge. Improvements in the manufacturing process result in a membrane with a higher density of nylon per unit area. The increased charge and greater nylon density provide increased binding sites for samples.

Pore size: 0,45 µm

Nytran® binding capacity: >600 µg/cm²
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