Coomassie® brilliant blue R-250 ~50% (dye content), Technical Grade, Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPYTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
Synonyms:
Brillant blue R
, Brilliant Blue R250
, Brilliant indocyanine 6B
, Brilliant Blue R
, Serva Blue R
, N-[4-[[4-[(4-Ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulphophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulpho-benzenemethanaminium inner salt, sodium salt (1:1)
, Coomassie Blue R 250
, Hydrogen [4-[4-(p-ethoxyanilino)-4'-[ethyl(m-sulphonatobenzyl)amino]benzhydrylene]cyclohexa-2,5-dien-1-ylidene](ethyl)(m-sulphonatobenzyl)ammonium monosodium salt
, Acid Blue 83
, Brilliant Cyanine 6B
M-Clarity™ quality level = MQ200
Brilliant Blue R (C.l. 42660) is a triaminotriarylmethane dye with two amino groups being benzylated and one phenylated. It is also known as brilliant indocyanine 6B and coomassie blue R250. Brilliant Blue R has been widely used for protein band detection after SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis).
- Its other applications include:
- as a protein stain on epoxy-embedded tissue sections
- for quantitative micro densitometry of protein in sections
- for staining chromatids, actinin microfilaments, developing neurites, and sperm acrosomes
- For detection of protein bands following electrophoresis
Formula:
C₄₅H₄₄N₃NaO₇S₂ MW: 825.98 g/mol Melting Pt: 174…180 °C Storage Temperature: Room Temperature |
MDL Number:
MFCD00041762 CAS Number: 6104-59-2 EINECS: 228-060-5 |
Specification Test Results
Appearance (Form) | Powder |
Suitability For Detection of Protein Bands Following Electrophoresis | Suitable |
Solubility (Colour) | Very Dark Purple to Very Dark Blue and Dark Blue-Black |
Carbon | 32.0 - 42.0 % |
Nitrogen | 2.5 - 3.5 % |
Dye Content | Approximately 50% |
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