4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) alkaline phosphatase substrate, Sigma-Aldrich®

Supplier: Merck
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Avg. Ratings: 0.0 out of 5


M-Clarity™ quality level = MQ200

P4744-10G P4744-1G P4744-25G P4744-50G P4744-5G
SIALP4744-10GEA 920 GBP
SIALP4744-10G SIALP4744-1G SIALP4744-25G SIALP4744-50G SIALP4744-5G
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) alkaline phosphatase substrate, Sigma-Aldrich®
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP)

Phosphatase substrate has been used as a substrate in tartrate-resistant acid phosphatase (TRAP) assay and alkaline phosphatase (ALP). Substrate for the determination of acid and alkaline phosphatases.


Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.

Formula: C₆H₄NNa₂O₆P·6H₂O
Melting Pt: 300 °C
Storage Temperature: Freezer
MDL Number: MFCD00066288
CAS Number: 333338-18-4
EINECS: 224-246-5

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