Nitro blue tetrazolium chloride (NBT), yellow powder

Supplier: MP Biomedicals
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Total Ratings: 0
Avg. Ratings: 0.0 out of 5

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Synonyms: 5,5'-Diphenyl-3,3'-bis(4-nitrophenyl)-2,2'-(3,3'-dimethoxybiphenyl-4,4'-ylene)ditetrazolium dichloride

ICNA0210041691EA 255 GBP
ICNA0210041691 ICNA0210041690 ICNA0210041694 ICNA0210041683 ICNA0210041650
Nitro blue tetrazolium chloride (NBT), yellow powder
Nitro blue tetrazolium chloride (NBT)

p-Nitroblue Tetrazolium Chloride is a substrate for dehydrogenases and other oxidases and precipitating agent for fibrinogen.


It is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.


Slightly (10 mg/ml) soluble in water.

Formula: C₄₀H₃₀Cl₂N₁₀O₆
MW: 817.65 g/mol
Melting Pt: 189…192 °C (lit.)
Storage Temperature: Refrigerator
MDL Number: MFCD00012159
CAS Number: 298-83-9
EINECS: 206-067-4

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Specification Test Results

identity Test Passes
l max (MeOH) 255 ± 3 nm

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