Nitro blue tetrazolium chloride (NBT), tablets, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: 5,5'-Diphenyl-3,3'-bis(4-nitrophenyl)-2,2'-(3,3'-dimethoxybiphenyl-4,4'-ylene)ditetrazolium dichloride

M-Clarity™ quality level = MQ200

N5514-10TABEA 102 GBP
N5514-10TAB N5514-25TAB
Nitro blue tetrazolium chloride (NBT), tablets, Sigma-Aldrich®
Nitro blue tetrazolium chloride (NBT)

Nitro Blue Tetrazolium is a tetrazole compound with two tetrazole rings.


  • Nitro Blue Tetrazolium has been used
  • To measure superoxide radicals
  • In lactate dehydrogenase quantification assay
  • For blot development in western blotting analysis
  • In NADPH-d histochemistry
  • As a reagent to analyse the properties of murine intestinal mucins by electrophoresis and histology in immunohistochemistry


Packaging: Contains 10 mg substrate per tablet.

Formula: C₄₀H₃₀Cl₂N₁₀O₆
MW: 817.65 g/mol
Melting Pt: 200 °C
Storage Temperature: Refrigerator
MDL Number: MFCD00012159
CAS Number: 298-83-9
EINECS: 206-067-4
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Specification Test Results

Appearance (Colour) Yellow
Diameter 5/32 inches
Form Round Tablet
Dissolution Time ≤5 min
Solubility (Colour) Faint Yellow to Yellow
Solubility (Turbidity) Clear
pH 4.5 - 5.5
Total Tablet Weight 21.2 - 28.8 mg/tab
Note pH Test,Dissolution time, and solubility are the results from one tablet in one ml of water.

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