Human IL-13 ELISA Kits
Supplier: STEMCELL TechnologiesTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
For detection and measurement of human interleukin 13.
The Human Interleukin 13 (IL-13) ELISA Kit is designed for the quantitative detection and measurement of IL-13 in biological fluids such as serum, plasma, and cell culture supernatants.
IL-13 is secreted by Th2 cells, mast cells and NK cells. IL-13 is involved in promoting B cell proliferation, induces class switching to IgG4 and IgE, recruits and activates IgE-producing B cells, and activates the JAK/STAT and IRS-1 and IRS-2 pathways. The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine.
The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase (SA-HRP), which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’, 5,5’ tetramethylbenzidine (TMB) results in a coloured product with an intensity directly proportional to the concentration of cytokine in the sample.
The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analysed in parallel.
Caution: Product restrictions: (i) Customer shall not make products or any portion of them in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier and (ii) Customer is familiar with Supplier’s restrictions on the use of the products and shall use the products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').
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