Crystal violet ≥90% (by anhydrous basis) ACS, Sigma-Aldrich®

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Synonyms: Methylrosanilide chloride, Basic violet 3, Viocid, N-(4-(Bis(4-(dimethylamino)phenyl)methylene)cyclohexa-2,5-dien-1-ylidene)-N-methylmethanamonium chloride, Violet crystal, Methyl violet 10B, Dimethyl((di(4-(dimethylamino)phenyl)methylen)-2,5-cyclohexadien-1-ylidene)ammonium chloride, CI 42555, 4-[4,4'-Bis(dimethylamino) benzhydrylidene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium chloride, Hexamethyl violet, Hexamethyl-p-rosaniline chloride, Vianin, Gentian violet

C6158-100G C6158-50G
C6158-100GEA 77.7 GBP
C6158-100G C6158-50G
Crystal violet ≥90% (by anhydrous basis) ACS, Sigma-Aldrich®
Crystal Violet

Crystal violet has been used to determine cell viability. It has been used in the staining of cells for the quantification of cellular migration.


Crystal violet can be used for DNA visualisation in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal violet helps in colorimetric measurement of cell viability.

Formula: C₂₅H₃₀ClN₃
MW: 407.99 g/mol
Melting Pt: 205 °C (dec., lit.)
Density: 1.19 g/cm³ (20 °C)
Storage Temperature: Room Temperature
MDL Number: MFCD00011750
CAS Number: 548-62-9
EINECS: 208-953-6
UN: 3077
ADR: 9,III
Merck Index: 13,04406
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Specification Test Results

Appearance (Colour) Green to Very Dark Green
Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Loss on Drying ≤ 7.5 %
Sensitivity
as Indicator
Pass
UV Absorbance Ratio 0.98 - 1.90
Purity ≥90.0 %
Meets ACS Requirements Current ACS Specification

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