Safranine O ≥85% (dye content), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: Safranin TSafranin Y3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chlorideSafranin OGossypimine3,7-Diamino-2,8-dimethyl-5-phenylphenazin-5-ium chlorideCI 50240Basic Red 2Cotton RedSafranin ASafranin

M-Clarity™ quality level = MQ200

S2255-100GEA 294 GBP
S2255-100G S2255-25G
Safranine O ≥85% (dye content), Sigma-Aldrich®
Safranine O

Safranin O is a cationic dye, which displays less significant metachromasia. It is used to detect any variations in articular disease. Safranin O binds specifically to polyanions. It detects the amount of proteoglycan present in cartilages. Safranin O is used as a marker for glycosaminoglycan depletion in osteoarthritis. Safranin is used as a counter stain in gram staining.


Safranin O has been used in the histological analysis of femurs. It has also been used to detect the presence of glycosaminoglycans in the inner body of meniscus structures.

Formula: C₂₀H₁₉ClN₄
MW: 350.85 g/mol
Density: 1.447 g/cm³ (22 °C)
Storage Temperature: Room Temperature
MDL Number: MFCD00011759
CAS Number: 477-73-6
EINECS: 207-518-8
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Specification Test Results

Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Nitrogen 13.6 - 16.8 %
Dye Content ≥ 85 %
Solubility (Colour) Dark Red to Very Dark Red
Solubility (Turbidity)
1 mg/mL, H2O
Clear
Wavelength 530 - 534 nm
E1%
50% EtOH and H2O
1250 - 1650
Recommended Retest Period 4 Years

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