Neutral red, powder, Bioreagent cell culture reagent, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: N2,N2,7-Trimethylphenazine-2,8-diamine hydrochlorideToluylene redCI 500403-Amino-7-dimethylamino-2-methylphenazineN8,N8,3-Trimethyl-2,8-phenazinediamine monohydrochlorideBasic Red 5

M-Clarity™ quality level = MQ200

N4638-1GEA 55.6 GBP
N4638-1G N4638-5G
Neutral red, powder, Bioreagent cell culture reagent, Sigma-Aldrich®
Neutral red

Neutral red (C.l. 50040, Cl Basic red 5) is a protonated diaminophenazine dye that has a small planar conjugated system.


  • Neutral red has been used in several histological staining applications that include:
  • As a nuclear stain
  • As a counterstain in the Gram procedure, either alone, or as a component of the Twort stain, a fast green-neutral red mixture
  • Fluorescent staining of nucleic acids in nervous tissue, of lipids and hydrophobic substances such as suberin and of phenolic compounds in algal cell walls
  • Detection of viable yeast cells in dandruff samples and of mussel larvae
  • Neutral red has also been used as a stain in flow cytometry
  • Useful as an indicator for preparing neutral red paper, and as a biological stain

Formula: C₁₅H₁₇ClN₄
MW: 288.78 g/mol
Melting Pt: 290 °C (dec., lit.)
Storage Temperature: Refrigerator
MDL Number: MFCD00012651
CAS Number: 553-24-2
EINECS: 209-035-8
Merck Index: 12,06571
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Specification Test Results

Appearance (Colour) Brown to Black
may have red or green sheen
Appearance (Form) Powder
Solubility (Colour)
10 mg/ml, H₂O
Dark Red
Infrared spectrum Conforms to Structure
Dye Content
(Calculated based on UV spectral assay)
≥90 %
Cell Culture Test Pass
Recommended Retest Period 4 years

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