Alcian blue solution, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: C.I. 74240C.N. AnAn-6Ingrain Blue 1Alcian blue 8 GX

M-Clarity™ quality level = MQ100

B8438-500MLEA 262 GBP
B8438-500ML B8438-250ML
Alcian blue solution, Sigma-Aldrich®
Alcian blue 8 GX

Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.


Alcian Blue is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby aiding in the quantitative determination of glycosaminoglycans.[5] It is also used for the staining of bone and cartilage.

Formula: C₅₆H₆₈Cl₄CuN₁₆S₄
MW: 1298.86 g/mol
Storage Temperature: Room Temperature
MDL Number: MFCD00010720
CAS Number: 33864-99-2

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Specification Test Results

Appearance (Colour) Dark Blue to Very Dark Blue
Appearance (Form)
Sediment free
Liquid
Wavelength
Absorbance
1:400 in H2O
0.45 - 0.60 Abs UN
pH 2.4 - 2.6

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