Crystal violet ethanolic aqueous solution primary stain for Gram staining, Sigma-Aldrich®

Supplier: Merck
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Synonyms: Methylrosanilide chloride, Basic violet 3, Viocid, N-(4-(Bis(4-(dimethylamino)phenyl)methylene)cyclohexa-2,5-dien-1-ylidene)-N-methylmethanamonium chloride, Violet crystal, Methyl violet 10B, Dimethyl((di(4-(dimethylamino)phenyl)methylen)-2,5-cyclohexadien-1-ylidene)ammonium chloride, CI 42555, 4-[4,4'-Bis(dimethylamino) benzhydrylidene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium chloride, Hexamethyl violet, Hexamethyl-p-rosaniline chloride, Vianin, Gentian violet

1.09218.0500 1.09218.2500
1.09218.0500EA 39.8 GBP
1.09218.0500 1.09218.2500
Crystal violet ethanolic aqueous solution primary stain for Gram staining, Sigma-Aldrich®
Crystal Violet

The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.


The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by safranin solution and appear orange.

Formula: C₂₅H₃₀ClN₃
MW: 407.99 g/mol
Storage Temperature: Ambient
MDL Number: MFCD00011750
CAS Number: 548-62-9
UN: 1170
ADR: 3,III
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