Papanicolaou's solution 3a polychromatic solution EA 31 used in nuclear staining, for cytology, Sigma-Aldrich®

Supplier: Merck
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1.09271.0500 1.09271.2500
1.09271.0500EA 52.7 GBP
1.09271.0500 1.09271.2500
Papanicolaou's solution 3a polychromatic solution EA 31 used in nuclear staining, for cytology, Sigma-Aldrich®
Papanicolaou's solution 3a polychromatic solution EA 31

Used to demonstrate the differentiation of squamous cells in gynecological cytology


The cytological staining technique developed by Papanicolaou is still the standard method in cancer and hormone cycle diagnosis. Four different polychrome solutions are available; they differ in the various concentrations of the components and produce different cytoplasm stains. In the last staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown. The polychrome solution is used to demonstrate the differentiation of squamous cells. In gynecological cytology Polychrome solution EA 31 (3a) and EA 50 (3b) are mostly used. In non-gynecological cytology Polychrome solution EA 65 (3c) and EA 65 (3d) are used. It is an IVD registered product and CE certified, thus can be used for clinical diagnostic purposes.

Density: 0.82 g/cm3 (20 °C)
Flash Pt: 17 °C
UN: 1993
ADR: 3,II
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Specification Test Results

Suitability for microscopy (Vaginal smear) passes test
Nuclei blue to dark violet
Cyanophilic cytoplasm blue-green to green
Eosinophilic cytoplasm pink

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