Haematoxylin modified solution Gill II used in nuclear staining, Sigma-Aldrich®

Supplier: Merck
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Synonyms: HydroxybrasilinDcH-48Natural Black I

1.05175.2500EA 186 GBP
1.05175.2500 1.05175.0500
Haematoxylin modified solution Gill II used in nuclear staining, Sigma-Aldrich®
Haematoxylin staining solutions

Hematoxylin solution modified acc. to Gill II


In hematoxylin-eosin staining (H&E), the most widely used histological staining technique, nuclear staining is achieved using ready-to-use staining solutions, such as Mayer’s hemalum solution or Hematoxylin solution modified according to Gill II or Gill III. These solutions do not need to be filtrated prior use and the dye is oxidized in a controlled manner, which renders the results robust and allows a brilliant differentiation of the stained structures. Counterstaining of i.e. for proteins, collagen, keratin, intercellular substances. can be performed with alcoholic or aqueous Eosin Y solution 0.5 %. A 500 ml bottle is sufficient for 1500 up to 2500 slides. It is registered as IVD and certified as CE product.

Formula: C₁₆H₁₄O₆
MW: 302.28 g/mol
Density: 1.04…1.06 g/cm3 (20 °C)
Storage Temperature: Ambient
MDL Number: MFCD00078111
CAS Number: 517-28-2
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Specification Test Results

Suitability for microscopy passes test
Nuclei (Vaginal smear) blue to dark violet
Cyanophilic cytoplasm bluish green to green
Eosinophilic cytoplasm pink

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