Haematoxylin modified solution Gill II used in nuclear staining, Sigma-Aldrich®
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Hematoxylin solution modified acc. to Gill II
In hematoxylin-eosin staining (H&E), the most widely used histological staining technique, nuclear staining is achieved using ready-to-use staining solutions, such as Mayer’s hemalum solution or Hematoxylin solution modified according to Gill II or Gill III. These solutions do not need to be filtrated prior use and the dye is oxidized in a controlled manner, which renders the results robust and allows a brilliant differentiation of the stained structures. Counterstaining of i.e. for proteins, collagen, keratin, intercellular substances. can be performed with alcoholic or aqueous Eosin Y solution 0.5 %. A 500 ml bottle is sufficient for 1500 up to 2500 slides. It is registered as IVD and certified as CE product.
Formula:
C₁₆H₁₄O₆ Density: 1.04…1.06 g/cm3 (20 °C) Storage Temperature: Ambient |
MDL Number:
MFCD00078111 CAS Number: 517-28-2 |
Specification Test Results
Suitability for microscopy | passes test |
Nuclei (Vaginal smear) | blue to dark violet |
Cyanophilic cytoplasm | bluish green to green |
Eosinophilic cytoplasm | pink |
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