MitoView™ 720 mitochondria dye, for fluorescence microscopy
Supplier: BiotiumTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.
- Cellular localization: Mitochondria
- For live or fixed cells: For live/intact cells
- Assay type/options: No-wash staining, Real-time imaging
- Cell permeability: Membrane permeant
- Abs/Em: 720/758 nm*
- Detection channel: Cy®5, Cy®7*
- Potential-dependent?: Partial (Dyes localize to the cytoplasm after mitochondrial depolarization, but still retain fluorescence.)
MitoView™ 720 is a near-infrared mitochondrial dye with absorbance/emission at 720/758 nm. While optimally detected using Cy®7 settings, the dye is bright enough to be imaged in the Cy®5 channel, and can be combined with visible red fluorescent probes. Mitochondrial localization is dependent on mitochondrial membrane potential; when membrane potential is disrupted the dye relocalizes to the cytoplasm but still retains fluorescence.
*While optimal for Cy®7 settings, MitoView™ 720 is bright enough to be imaged in the Cy®5 channel.
Cy Dye is a registered trademark of GE Healthcare. MitoTracker is a registered trademark of Thermo Fisher Scientific.
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