Methylene blue (Loeffler) counterstain, for bacteriology, Sigma-Aldrich®

Supplier: Merck
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Synonyms: Basic Blue 9, Methylthioninium chloride, Methylene blue chloride, 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride, Swiss Blue

1.01287.0100 1.01287.0500 1.01287.2500 1.01287.0100DP
1.01287.0100EA 32.8 GBP
1.01287.0100 1.01287.0500 1.01287.2500 1.01287.0100DP
Methylene blue (Loeffler) counterstain, for bacteriology, Sigma-Aldrich®
Methylene blue

Löffler's methylene blue solution is generally used as bacteriological counter staining of, e.g. of gonococcae, lactic acid bacteria and for visualization of the pole corpuscles of pasteurella.


It is often used in combination with Ziehl-Neelsen carbol fuchsin solution and hydrochloric acid in ethanol to stain mycobacteria. Löffler's methylene blue solution is an IVD product and CE registered, thus, it can be used in diagnostics and for laboratory accreditations.

Formula: C₁₆H₁₈ClN₃S
MW: 319.86 g/mol
Density: 0.97 g/cm3 (20 °C)
Flash Pt: 40 °C
Storage Temperature: Ambient
MDL Number: MFCD00150008
CAS Number: 61-73-4
UN: 1993
ADR: 3,III
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