FLUO 3/AM ≥95% (by HPLC) fluorescent indicator, for calcium determination

Supplier: Biotium
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Synonyms: 4-(6-Acetoxymethoxy-2,7-dichloro-3-oxo-9-xanthenyl)-4′-methyl-2,2′(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester1-[Amino-5-(2,7-dichloro-6-acetomethoxy-3-oxo-3H-xanthen-9-yl)phenoxy]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid pentaacetoxy-methyl ester

50013.EA 395 GBP
50013. 50014. 50016.
FLUO 3/AM ≥95% (by HPLC) fluorescent indicator, for calcium determination
FLUO 3/AM

Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening.


  • Abs/Em (after hydrolysis): 506/526 nm (low or high [Ca2 ] )
  • Extinction Coefficient (after hydrolysis): 86,000 M-1 cm-1 (506 nm)
  • Kd (Ca2 ) (after hydrolysis): 450 nM
  • Cell permeability: Membrane permeant
  • Orange red solid soluble in DMSO
  • Store -20°C and protect from light, especially when in solution


Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate dye loading. See the product information sheet for more information.

Formula: C₅₁H₅₀Cl₂N₂O₂₃
MW: 1129.85 g/mol
Storage Temperature: Freezer
CAS Number: 121714-22-5

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