Silylation derivatisation reagents
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Silylation is the most widely used derivatisation procedure for sample analysis by GC. In silylation, an active hydrogen is replaced by an alkylsilyl group such as trimethylsilyl (TMS) or tert-butyldimethylsilyl (tert-BDMS). Silyl derivatives are more volatile, less polar, and more thermally stable. As a result, GC separation is improved and detection is enhanced.
- Replace active hydrogen, reducing polarity and making the compounds more volatile
- Increase stability of derivatives
Both TMS and tert-BDMS reagents are suitable for a wide variety of compounds and can be used for many GC applications.
Caution: Note that silylation reagents are generally moisture-sensitive and must be sealed to prevent deactivation.
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