Crystal violet solution Grams for microscopy, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: Methylrosanilide chloride, Basic violet 3, Viocid, N-(4-(Bis(4-(dimethylamino)phenyl)methylene)cyclohexa-2,5-dien-1-ylidene)-N-methylmethanamonium chloride, Violet crystal, Methyl violet 10B, Dimethyl((di(4-(dimethylamino)phenyl)methylen)-2,5-cyclohexadien-1-ylidene)ammonium chloride, CI 42555, 4-[4,4'-Bis(dimethylamino) benzhydrylidene]cyclohexa-2,5-dien-1-ylidene]dimethylammonium chloride, Hexamethyl violet, Hexamethyl-p-rosaniline chloride, Vianin, Gentian violet

M-Clarity™ quality level = MQ200

94448-2.5L-F 94448-2.5L-FDP
94448-2.5L-FEA 91.9 GBP
94448-2.5L-F 94448-2.5L-FDP
Crystal violet solution Grams for microscopy, Sigma-Aldrich®
Crystal Violet

Gram’s crystal violet solution, also known as gentian violet or hexamethyl pararosaniline, is a basic, cationic, acidotropic aminoarylmethane dye. It is a hexa-N-methylated triaminotriphenylmethane with a moderately sized lipophilic cation and a slightly nonplanar conjugated system. It is used in bacteriology as the primary stain in Gram staining to differentiate Gram-positive and Gram-negative bacteria. It is known to possess antibacterial and antifungal properties.


  • Crystal violet is routinely used for Gram staining and its variants, staining amyloid, bacterial components, and vascular plant tissues
  • It is used in polychrome staining of epoxy resin sections, viability staining of cultured neurons, and confocal optical sectioning to analyse meiotic structures
  • It is also employed in the acridine orange-crystal violet staining of intracellular bacteria, microsporidian spores, and cytological smears


The antimicrobial effects of crystal violet are caused by free radical generation and the formation of unionised dye-bacteria complexes. It also inhibits bacterial protein synthesis and cell wall formation and is especially effective against Gram-positive bacteria.

Formula: C₂₅H₃₀ClN₃
MW: 407.99 g/mol
Density: 0,981 g/ml (20 °C)
Flash Pt: 34 °C
Storage Temperature: Room Temperature
MDL Number: MFCD00011750
CAS Number: 548-62-9
UN: 1170
ADR: 3,II

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Specification Test Results

Appearance (colour) Purple to very dark purple
Appearance (form) Liquid
Thin layer chromatogr. Corresponds to standard
Density D20/4 0.970 - 0.985
Wavelength (1) (UV) 585 - 595 nm
Extinction (1%/1 cm) (1) (UV) Report result
Solvent (UV) Water
Expiry period 36 months

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