Bis(2-hydroxyethyl)amino tris(hydroxymethyl)methane (BIS-TRIS) ≥99% (by titrimetric analysis), white powder

Supplier: MP Biomedicals
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0210103825 0210103880 0210103890 0210103891
ICNA0210103825EA 51.6 GBP
ICNA0210103825 ICNA0210103880 ICNA0210103890 ICNA0210103891
Bis(2-hydroxyethyl)amino tris(hydroxymethyl)methane (BIS-TRIS) ≥99% (by titrimetric analysis), white powder
Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane (BIS-TRIS)

Bis-Tris is zwitterionic buffer, an amine buffer similar to Tris. It exhibits only a very small shift in dissociation constant with respect to temperature. Hydrochloric acid can be used to adjust pH for a Bis-Tris buffer to the desired pH.


  • Presentation: White Powder
  • Extinction Coefficient (E1%): 259 nm = 10. 277 nm = 10 (water)
  • pKa: (25 °C) 6.5
  • EM (water): 259 nm = 10; 277 nm = 10
  • Soluble in water (1 M - clear, colorless solution).
  • Storage temperature: Room Temperature


pH: Useful pH range 5.8-7.2

Formula: C₈H₁₉NO₅
Melting Pt: 101…106 °C
Storage Temperature: Ambient
MDL Number: MFCD00002853
CAS Number: 6976-37-0

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Specification Test Results

Identity Test Passes
Purity ≥99%
FTIR Conforms to Standard
pH (1% aq soln) 9-10
Moisture ≤1%

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