Fluor phosphine reagents, DyLight™, Pierce™
Supplier: Thermo Fisher ScientificTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
DyLight™ Fluor phosphine reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.
- Easily dissolves in water-miscible solvents (eg. DMSO) for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
- Reaction chemistry occurs effectively in simple buffer conditions requiring no accessory reagents such as copper or reducing agents, and does not interfere with fluorescence applications
- The phosphine reactive group is specific in biological samples for bioorthogonal azide-tagged molecules, ensuring that fluorescent labeling is specific
- DyLight™ 488, 550 and 650 are intense, highly stable fluorophores for green, orange and red fluorescent detection
When used in combination with azide labeling strategies, phosphine-activated dyes enable selective fluorescent labeling for detection of protein interactions and post-translational modifications using fluorescence imaging technologies. The phosphine group conjugates to azide groups by the Staudinger reaction mechanism.
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