Fluor phosphine reagents, DyLight™, Pierce™

Supplier: Thermo Fisher Scientific
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Fluor phosphine reagents, DyLight™, Pierce™
Protein Linking and Labeling Systems

DyLight™ Fluor phosphine reagents are phosphine-activated fluorescent dyes for specific labeling and detection of azide-tagged molecules, which enables use of fluorescence imaging in metabolic labeling strategies.


  • Easily dissolves in water-miscible solvents (eg. DMSO) for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
  • Reaction chemistry occurs effectively in simple buffer conditions requiring no accessory reagents such as copper or reducing agents, and does not interfere with fluorescence applications
  • The phosphine reactive group is specific in biological samples for bioorthogonal azide-tagged molecules, ensuring that fluorescent labeling is specific
  • DyLight™ 488, 550 and 650 are intense, highly stable fluorophores for green, orange and red fluorescent detection


When used in combination with azide labeling strategies, phosphine-activated dyes enable selective fluorescent labeling for detection of protein interactions and post-translational modifications using fluorescence imaging technologies. The phosphine group conjugates to azide groups by the Staudinger reaction mechanism.

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