Plasmid Screening ToothPick™ and ToothPick™-PCR
Supplier: G BiosciencesTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
Plasmid Screening ToothPick™ allows the rapid screening of bacteria for transformed plasmids. Simply pick a colony, add to Plasmid Screening ToothPick™ reagents and then analyse using restriction enzymes.
- Simple protocol
- No overnight cultures required
ToothPick™-PCR is an extension of ToothPick™ and allows for the rapid release of plasmids from transformed bacteria for screening by PCR. There is no requirement for growing bacteria, performing 'minipreps' or purifying the plasmid DNA. Add your colony directly to the supplied ToothPick™ solution, heat and then transfer 1 μl directly to a PCR reaction to screen for your plasmid. Following PCR, add the supplied GLOW™ Loading Dye and run on an agarose gel. No need to stain with ethidium bromide, simply place gel on UV box and view. Glow Dyes™ have been specifically formulated for nucleic acid electrophoresis and contain an optimised concentration of buffer agents, tracking dyes and ethidium bromide. These loading dyes reduce exposure and many of the problems associated with ethidium bromide use and disposal.
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