VWR®, PCR buffers
Supplier: VWR ChemicalsTotal Ratings: 0
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An optimal buffer system is critical for the performance of successful PCR. VWR® Taq DNA Polymerase kits generally include two different buffers, Key Buffer and Extra Buffer, which are suited for different PCR needs. All buffers contain Tris and 15 mM MgCl₂ (1,5 mM MgCl₂ final concentration). Additional MgCl₂ for easy optimisation is included in a separate vial.
Key Buffer: Key Buffer (NH⁴⁺) gives a superior amplification signal (high yield) and minimises the need for optimisation of the Mg²⁺ concentration or the annealing temperature in most primer-template systems.
Extra Buffer: Extra Buffer is the traditional potassium (K+) buffer. Extra Buffer promotes high specificity. Careful optimisation of primer annealing temperatures and Mg²⁺ concentrations may be required.
Combination Buffer: Combination Buffer is a proprietary mixture of K+ and NH⁴⁺. It combines high specificity with good product yield and high tolerance to optimisation of primer annealing temperatures and Mg²⁺ concentrations due to its balanced ammonium-potassium formulation.
Buffers for GC-rich templates: Combined with VWR® TEMPase the GC buffers promote excellent amplification results with targets of varying degrees of GC content. This confirms that the choice of buffer is crucial for any successful amplification.
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