Trypan blue 60% (dye content) ≥80% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: Tolidine-disazo-bis-8-amino-1-naphthol-3,6-disulphonic acid tetrasodium salt3,3′-Dimethyl-4,4′-bis(5-amino-4-hydroxy-2,7-disulphonaphthyl-3-azo)-[1,1′-biphenyl] tetrasodium saltCI 23850Direct Blue 14AzidinBlueSodium 3,3'-((3,3'-dimethyl-[1,1'-biphenyl]-4,4'-diyl)bis(diazene-2,1-diyl))bis(5-amino-4-hydroxynaphthalene-2,7-disulphonate)Benzamine blueTrypan blue sodium saltDiphenyl Blue

M-Clarity™ quality level = MQ200

302643-25GEA 39.2 GBP
302643-25G 302643-100G
Trypan blue 60% (dye content) ≥80% (by HPLC), Sigma-Aldrich®
Trypan Blue

Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B. Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

Formula: C₃₄H₂₄N₆Na₄O₁₄S₄
MW: 960.82 g/mol
Melting Pt: >300 °C (lit.)
Storage Temperature: Room Temperature
MDL Number: MFCD00003969
CAS Number: 72-57-1
EINECS: 200-786-7

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Specification Test Results

Appearance (Color) Dark Green to Brown or Brown-Black
Appearance (Form) Powder or Crystals or Granules
Infrared spectrum Conforms to Structure
Wavelength 603 - 607 nm
Extinction Coefficient, c = 0.01 g/L; Methanol ≥60000
Carbon ≥24.6 %
Nitrogen ≥5.0 %
Purity (HPLC) ≥80.0 %
Solubility (Turbidity); Opaque, c = 1mg/ml; Water Conforms
Solubility (Color) Dark Blue
Recommended Retest Period 5 Years

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