Trypan blue 0,4% in aqueous solution, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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M-Clarity™ quality level = MQ200

93595-250ML 93595-50ML
93595-250MLEA 106 GBP
93595-250ML 93595-50ML
Trypan blue 0,4% in aqueous solution, Sigma-Aldrich®
Trypan Blue

Trypan blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.


  • it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents
  • employed as a collagen stain in a Van Gieson procedure
  • To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains
  • used as a fluorescent tracer of cell populations in embryology
  • used as a tumor promoter modulating permeability of lysosomal membranes


Physical form: 0,4% in 0,81% sodium chloride; 0,06% potassium phosphate, dibasic.

Formula: C₃₄H₂₄N₆Na₄O₁₄S₄
Density: 1,007 g/ml at 20 °C
Storage Temperature: Room Temperature
MDL Number: MFCD00003969
CAS Number: 72-57-1
EINECS: 200-786-7
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Specification Test Results

Appearance (colour) Blue to very dark blue
Appearance (form) Liquid
Thin layer chromatogr. Corresponds to standard
Density d20/4 0.997 - 1.017
Wavelength (1) (UV) 600 - 610 nm
Extinction (1%/1 cm) (1) (UV)
Solvent (UV) Methanol
Expiry period 30 months

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