Isolate DNA from plant or fungal sample with organic solvents using spin columns.
The E.Z.N.A.® High Performance (HP) Plant & Fungal DNA Kit is designed for efficient recovery of genomic DNA from fresh, frozen, and dried plant or fungal tissue samples rich in polysaccharides or having lower DNA content. Up to 100 mg wet tissue (or 50 mg dry tissue) can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant or fungal tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques.
If using the E.Z.N.A.® HP Plant & Fungal DNA Kit for the first time, please read this booklet to become familiar with the procedures. This procedure relies on the well established properties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), in conjunction with the selective DNA binding of Omega Bio-tek’s HiBind® matrix, to purify high-quality DNA. Samples are homogenized and lysed in a high salt buffer containing CTAB and extracted with chloroform to remove polysaccharides and other components that interfere with many routine DNA isolations and downstream applications. Binding conditions are adjusted and DNA is further purified using HiBind ® DNA Mini Columns. In this way, salts, proteins, and other contaminants are removed to yield high-quality genomic DNA suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization.
