Osmium tetraoxide 4% in aqueous solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY
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Synonyms: Osmium (VIII) oxide, Osmic acid anhydride, Osmic acid

M-Clarity™ quality level = MQ100

75632-10ML 75632-5ML
75632-10MLEA 326 GBP
75632-10ML 75632-5ML
Osmium tetraoxide 4% in aqueous solution, Sigma-Aldrich®
Osmium tetraoxide

Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.


Osmium tetroxide solution has been used as a fixative in scanning and transmission electron microscopy.

Formula: O₄Os
MW: 254.23 g/mol
Boiling Pt: 130
Melting Pt: 40
Density: 1,0 g/ml at 20 °C
Storage Temperature: Refrigerator
MDL Number: MFCD00011150
CAS Number: 20816-12-0
EINECS: 244-058-7
UN: 3287
ADR: 6.1 II
Merck Index: 13,06962
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Specification Test Results

Assay 4.0% OsO₄ in water


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