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You Searched For: Electroporators

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Cell culture

Cell culture Both autologous and allogeneic cells are obtained from donors utilizing methods including but not limited to apheresis aspirates and surgical biopsies. The donor cells of interest are processed to remove undesirable contaminants and p...

Cell Biology Products, Supplies, and Resources | VWR

Cell Biology Cells are the fundamental unit of life. When you are focused on cell biology Avantor is your source for the tools products and supplies you need to keep your lab functioning at its peak. You can be concerned with the biochemical and p...

Transfect

Transfect VWR offers transfection reagents for numerous applications including bioproduction in vivo work viral production DNA and siRNA transfection CRISPR/Cas 9 and more. In addition to transfection reagents electroporation equipment supporting ...

细胞疗法的上游流程

细胞疗法的上游流程 与其他生物制剂相关的生产量(例如,单克隆抗体:2000L至10000L)相比,细胞疗法的生产量通常在1L至10L之间。 即使质量参数也不同 细胞疗法不是争取最大的效价和蛋白质量,而是着眼于最大的细胞给药剂量和所施用的细胞/组织的功能。 因此,培养基,补充剂,生物反应器系统和起始细胞质量等上游生产参数非常重要。 最后,在完成培养过程后,需要使用不影响细胞产品质量的细胞解离试剂收获治疗细胞。 探索细胞疗法上游流程的解决方案 Avantor Seradigm血清 Cell c...

~~27806 LabSet-Up-Guide 2021 Genomics checklist~~

LABORATORY CHECKLIST Genomics ☐ Plasmid Purification Standard High Purity Plasmid Isolation Rapid Plasmid Isolation Endotoxin-Free Plasmid Isolation Yeast Plasmid Isolation Colony Screening Column Regeneration Accessories ☐ Genomic DNA Purificatio...

单克隆抗体的上游工艺

单克隆抗体的上游工艺 开发和制造单克隆抗体已经摆脱了对动物原材料的依赖。我们的生化试剂不含动物成分,以满足这些应用的需要。 为单克隆抗体上游工艺提供的产品用于支持细胞生长,同时满足所需的质量和性能参数。这使得从工艺开发到中试,再到最终的商业制造,实现了无缝过渡。 随着您的流程不断扩大,我们的供应链管理系统可以轻松处理增加的材料数量。一旦您确定了最佳的内部开发生产用基础培养基,我们可以为您定制液体或干燥形式的培养基,并将其包装起来,以便有效地转移到您的生物反应器中。 探索单克隆抗体上游工艺的...

Gene Editing Technology, Tools and Resources

Gene Editing Technology Tools and Resources Gene Editing is the application area concerned with the intentional alteration of the DNA sequence of a plasmid cell or organism. Gene editing encompasses numerous methods including mutagenesis restricti...

Upstream Gene Therapy Products & Solutions in Bioprocessing

Upstream Gene Therapy Products & Solutions in Bioprocessing Achieve optimal generation of gene therapy vectors with our non-viral transfection and viral transduction products. Avantor® can provide culture systems that support processes development...

細胞培養

細胞培養 自家細胞と同種異系細胞の両方が、アフェレーシス、吸引物、および外科的生検を含むがこれらに限定されない方法を利用するドナーから得られる。 目的のドナー細胞は、望ましくない汚染物質を除去するために処理され、細胞培養手順のために準備されます。 最終的な細胞産物に必要な数を増やすために、ドナー細胞は、サイトカイン、成長因子、および必須アミノ酸とビタミンを補充した培地で培養されます。 付着依存性ドナー細胞用に設計されたバイオリアクターシステムも使用されます。 多くのドナー細胞は、動物の血清...

Microsoft Word - GeCKOv2.0 library amplification protocol.docx

Microsoft Word - GeCKOv2.0 library amplification protocol.docx   Page 1 of 3 rev20140502 Genome-scale CRISPR Knock-Out (GeCKO) v2.0 pooled libraries CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a microbial nuclease system ...

Upstream processing for cell therapy

Upstream processing for cell therapy Compared to production volumes associated with other biologics (e.g. mAbs: 2000L to 10000L) cell therapy production volumes are more commonly between 1L to 10L. Even the quality parameters are different. Instea...

Upstream processing for cell therapy

Upstream processing for cell therapy Compared to production volumes associated with other biologics (e.g. mAbs: 2000L to 10000L) cell therapy production volumes are more commonly between 1L to 10L. Even the quality parameters are different. Instea...

Microsoft Word - GeCKOv2.0 library amplification protocol.docx

Microsoft Word - GeCKOv2.0 library amplification protocol.docx   Page 1 of 3 rev20140502 Genome-scale CRISPR Knock-Out (GeCKO) v2.0 pooled libraries CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is a microbial nuclease system ...
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