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You Searched For: Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.


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Image Unavailable-ORIGMG225346

Slc7a11 (NM_011990) mouse tagged ORF clone

Catalog Number: (ORIGMG225346)
Supplier: OriGene
Description: Slc7a11 (GFP-tagged) - Mouse solute carrier family 7 (cationic amino acid transporter y+ system) member 11 (Slc7a11), (10 µg).
UOM: 1 * 10 µG
Image Unavailable-69909-3

pET-30a(+) DNA, Novagen®

Catalog Number: (69909-3)
Supplier: Merck Millipore (Novagen)
Description: The pET-30a-c(+) vectors carry an N-terminal His Tag®/thrombin/S Tag™/enterokinase configuration plus an optional C-terminal His tag sequence.
UOM: 1 * 10 µG

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pSTBlue-1 AccepTor™ vector kit

Supplier: Merck Millipore (Novagen)
Description: The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant <i>Taq</i> polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues<i> in vivo</i> following transformation.

Product Image-69750-3

pET-24b(+) DNA, Novagen®

Catalog Number: (69750-3)
Supplier: Merck Millipore (Novagen)
Description: The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).
UOM: 1 * 10 µG
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Expression vectors, pGEX

Supplier: Cytiva
Description: The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

Product Image-PIER16152

Expression vectors, green renilla Luc vector for luciferase assays, Pierce™

Catalog Number: (PIER16152)
Supplier: Thermo Fisher Scientific
Description: Green <i>Renilla</i> Luc vectors contain a gene cloned from the sea pansy, <i>Renilla reniformis</i>
UOM: 1 * 10 µG
Product Image-PIER16190

Pierce™ Gaussia-Dura Luc Vector for Luciferase Assays, Expression Vectors

Supplier: Thermo Fisher Scientific
Description: <i>Gaussia</i> Dura Luc vectors contain a mutant form of the <i>Gaussia</i> luciferase gene that confers better bioluminescent signal stability than native luciferase. <i>Gaussia</i>-Dura luciferase (approx. 20 kDa) is a secreted protein, which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

Product Image-27-1524-01

M13KO7 Helper Phage, Cytiva

Catalog Number: (27-1524-01)
Supplier: Cytiva
Description: M13 phage for the GST expression system.
UOM: 1 * 100 µl
Product Image-27-1542-01

E. coli BL21

Catalog Number: (27-1542-01)
Supplier: Cytiva
Description: The <i>E. coli </i>BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.
UOM: 1 * 1 Vial
Image Unavailable-ICNA04821226

Vector, pUC 18, MP Biomedicals

Catalog Number: (ICNA04821226)
Supplier: MP Biomedicals
Description: pUC 18 is a small vector of approximately 2,7 Kb containing the Pvu II/EcoR I fragment of pBR322 and the lacZ gene multiple cloning site from M13mp18.
UOM: 1 * 25 µG
Image Unavailable-ICNA04821225

Vector, pBR322, MP Biomedicals

Catalog Number: (ICNA04821225)
Supplier: MP Biomedicals
Description: pBR322 is a 4363 bp vector purified from <i>Escherichia coli</i>. It is one of the most commonly used cloning vectors, that confers resistance to ampicillin and tetracycline. It contains single restriction sites for Pst I and Pvu I within the ampicillin-resistance gene (ampr) and single restriction sites for BamH I, Sal I, Sph I, EcoR V, Nru I and Xma III within the tetracycline-resistance gene (tetr).
UOM: 1 * 25 µG
Product Image-PIER16150

Expression vectors, cypridina Luc vector for luciferase assays, Pierce™

Supplier: Thermo Fisher Scientific
Description: <i>Cypridina</i> Luc vectors contain a gene cloned from the marine ostracod, <i>Cypridina noctiluca.</i>

Product Image-27-4110-02

Polyadenylic acid (poly-A)

Supplier: Cytiva
Description: Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.

Certificates

Product Image-PIER16156

Expression vectors, red firefly Luc vector for Luciferase assays, Pierce™

Supplier: Thermo Fisher Scientific
Description: Red Firefly Luc vectors contains a mutant form of the firefly luciferase gene that has a red-shifted emission spectrum.

Product Image-PIER16148

Gaussia Luc vector for luciferase assays, expression vectors, Pierce™

Supplier: Thermo Fisher Scientific
Description: <i>Gaussia</i> Luc vectors contain a gene cloned from the copepod, <i>Gaussia princeps</i>. The gene encodes a naturally-secreted bioluminescent <i>Gaussia</i> luciferase (approx. 20 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

Image Unavailable-SIALD4154-10UG

PUC18 plasmid DNA

Catalog Number: (SIALD4154-10UG)
Supplier: Merck
Description: Plasmids are isolated from host strain <i>E. coli </i>RRI. These plasmids confer ampicillin resistance and complement defects in β-galactosidase in appropriate host strains.
UOM: 1 * 10 µG
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