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You Searched For: Sanger Sequencing Reagents

Sequencing reagents facilitate the processes when determining the precise order of nucleotides within DNA strands through sorting the fragments based on size. Enzymes are optimized to work together for high-efficiency removal of unincorporated primers and nucleotides. These reagents produce exceptional sequencing results from difficult templates, such as those with high GC content and secondary structures. Essential for basic and applied research procedures, sequencing reagents allow researchers to discover genotypes and phenotypes of specimen.

Sequencing reagents facilitate the processes when determining the precise order of nucleotides within DNA strands through sorting the fragments based on size. Enzymes are optimized to work together for high-efficiency removal of unincorporated primers and nucleotides. These reagents produce exceptional sequencing results from difficult templates, such as those with high GC content and secondary structures. Essential for basic and applied research procedures, sequencing reagents allow researchers to discover genotypes and phenotypes of specimen.


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Product Image-MAG-FRAG-I-5

Fragment selection kits, Axygen® AxyPrep™ FragmentSelect-I Kit

Catalog Number: (MAG-FRAG-I-5)
Supplier: Corning
Description: The AxyPrep™ FragmentSelect Kits utilise a paramagnetic bead technology for quick high throughput optimised DNA size selection suitable for various next generation sequencing platforms. The AxyPrep™ FragmentSelect-I kit is optimised for Illumina next generation sequencing platforms and Life Technologies (SOLiD™) fragment size selection needs simplifying the library construction workflow.
UOM: 1 * 1 KIT
Product Image-AATB17062

MagaDye™ 561 ddATP

Supplier: AAT BIOQUEST INC
Description: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

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Product Image-AATB17208

ddTTP [2',3'-Dideoxythymidine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)

Catalog Number: (AATB17208)
Supplier: AAT BIOQUEST INC
Description: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
UOM: 1 * 1 µmol

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Product Image-AATB17210

ddGTP [2',3'-Dideoxyguanosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)

Catalog Number: (AATB17210)
Supplier: AAT BIOQUEST INC
Description: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
UOM: 1 * 1 µmol

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Product Image-AATB17064

MagaDye™ 613 ddCTP

Supplier: AAT BIOQUEST INC
Description: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

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Product Image-AATB17080

2-Aminoethoxypropargyl ddCTP (1 µmole)

Catalog Number: (AATB17080)
Supplier: AAT BIOQUEST INC
Description: Sanger method is one of the most reliable and earliest DNA sequencing methods.
UOM: 1 * 1 µmol

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Product Image-AATB17063

MagaDye™ 535 ddGTP

Supplier: AAT BIOQUEST INC
Description: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

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Product Image-AATB17207

ddCTP [2',3'-Dideoxycytidine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)

Catalog Number: (AATB17207)
Supplier: AAT BIOQUEST INC
Description: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
UOM: 1 * 1 µmol

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Product Image-AATB17065

MagaDye™ 588 ddTTP

Supplier: AAT BIOQUEST INC
Description: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.

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Product Image-AATB17086

2-Aminoethoxypropargyl ddGTP (1 µmole)

Catalog Number: (AATB17086)
Supplier: AAT BIOQUEST INC
Description: Sanger method is one of the most reliable and earliest DNA sequencing methods.
UOM: 1 * 1 µmol

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Product Image-AATB17082

2-Aminoethoxypropargyl ddTTP (1 µmole)

Catalog Number: (AATB17082)
Supplier: AAT BIOQUEST INC
Description: Sanger method is one of the most reliable and earliest DNA sequencing methods.
UOM: 1 * 1 µmol

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Product Image-AATB17084

2-Aminoethoxypropargyl ddATP (1 µmole)

Catalog Number: (AATB17084)
Supplier: AAT BIOQUEST INC
Description: Sanger method is one of the most reliable and earliest DNA sequencing methods.
UOM: 1 * 1 µmol

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Product Image-AATB17209

ddATP [2',3'-Dideoxyadenosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)

Catalog Number: (AATB17209)
Supplier: AAT BIOQUEST INC
Description: Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
UOM: 1 * 1 µmol

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Image Unavailable-786-11

Arginine-C™, sequencing grade

Catalog Number: (786-11)
Supplier: G-Biosciences
Description: SG-Arginine-C™ endopeptidase (Clostripain, from <i>C. histolyticum</i>) specifically hydrolyses the carboxy peptide bond of arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilise enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.
UOM: 1 * 2 items
Image Unavailable-786-13

Chymotrypsin™, sequencing grade

Catalog Number: (786-13)
Supplier: G-Biosciences
Description: SG-Chymotrypsin™ is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It is therefore recommended to always use the shortest digestion time possible.
UOM: 1 * 2 items
Image Unavailable-786-15

Glutamic-C™, sequencing grade

Catalog Number: (786-15)
Supplier: G-Biosciences
Description: SG-Glutamic-C™ is a serine endopeptidase, from<i> S. aureus V8</i>, that is highly specific for the cleavage of peptide bonds at the carboxy side of either aspartic or glutamic acid, depending on the buffer used. In Tris-HCl buffer, in particular in the absence of phosphate ions, the enzyme is specific for the glutamyl site. Recommended buffers for fragmentation of proteins using this enzyme are 50 mM Tris-HCI, pH 8,0 or bicarbonate buffer. Highly purified preparations of SG-Glutamic-C™ are chemically modified making the enzyme both resistant to autolysis and stabilises its enzymatic activity.
UOM: 1 * 2 items
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
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