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Product Image-786-273

Antibody fab fragmentation kit

Description: Antibody Fab Fragmentation Kits are designed for the generation and isolation of Fab fragments from IgG molecules. The kits utilise Immobilised Papain resin. Immobilised Papain offers the advantage of generating Fab and fragments without the need to remove the papain enzyme after digestion. Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected.
Catalog Number: 786-273
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-786-275

Antibody F(ab)₂ fragmentation kits

Description: The F(ab)₂ Fragmentation kits are designed for the generation and isolation of F(ab)₂ fragments from IgG molecules. The kit utilises Immobilised Pepsin resin. Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)₂ fragments from immunoglobulin G (IgG). One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved, so the two Fab regions of the antibody remain joined together, yielding a divalent molecule (containing two antibody binding sites), hence the designation F(ab)₂. The light chains remain intact and attached to the heavy chain, whereas the Fc fragment is digested into small peptides. The Immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.
Catalog Number: 786-275
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-PIER89821

Complete bacterial protein extraction reagent, B-PER™

Description: B-PER™ Complete Bacterial Protein Extraction Reagent is an all-in-one formulation of non ionic detergent and enzymes that enables mild extraction of proteins from both gram-negative and gram-positive bacteria without the need for mechanical disruption. B-PER Complete Reagent combines a universal nuclease and lysozyme to improve cell lysis and reduce viscosity, and may be used for soluble protein extraction and inclusion body purification from bacterial cell lysates.
Catalog Number: PIER89821
UOM: 1 * 250 mL
Supplier: Thermo Fisher Scientific
Image Unavailable-786-129

SDS detection and estimation reagent kit

Description: A reagent kit for detection and estimation of SDS in a sample. Mix the test sample in the extraction buffer reagents provided with the kit. If SDS is present in the sample, a blue colour is extracted that can be quantitatively measured.
Catalog Number: 786-129
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-PIER88279

Mouse embryonic fibroblast isolation kit, Pierce™

Description: The Pierce™ Mouse Embryonic Fibroblast (MEF) Isolation Kit enables high yield isolation and culture of viable primary fibroblasts from mouse embryos.
Catalog Number: PIER88279
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Product Image-786-018

Protein fractionation kit, FOCUS™ Global Fractionation

Description: FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.
Catalog Number: 786-018
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-786-246

Proteome kits, mammalian proteome, FOCUS™

Description: Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.
Catalog Number: 786-246
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-786-249

Protein fractionation kit, FOCUS™ Membrane Proteins

Description: FOCUS™ Membrane Proteins for the fractionation of highly enriched membrane proteins.
Catalog Number: 786-249
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-786-257

FOCUS™ Yeast proteome kits

Description: Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilises nearly all of the proteins from yeast, including membrane and soluble proteins. Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™ Zymolyase®, which has improved stability and shelf life. Enzymatic action is followed by extraction of total proteome with the supplied strong chaotropic extraction buffer that solubilises even the most difficult proteins.
Catalog Number: 786-257
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-786-277

Antibody Fab and F(ab)₂ fragmentation of mouse IgG₁ kits

Description: Antibody Fab and F(ab)₂ Fragmentation of Mouse IgG<sub>1</sub> Kits are designed for the generation and isolation of Fab and F(ab)₂ fragments from mouse IgG₁ molecules. The kit utilises Immobilised Ficin resin. Ficin (or Ficain) (~25000 Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. Ficin has an effective range of pH 4 to 9,5 with an optimal pH of 6,5 and cleaves bonds that involve uncharged or aromatic amino acids. Ficin is typically used to cleave mouse IgG₁ as this is difficult to cleave with papain and pepsin. In the presence of 1 to 4 mM or 10 to 20 mM cysteine, ficin generates F(ab)₂ and Fab fragments respectively.
Catalog Number: 786-277
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-PIER78260

Bacterial Protein Extraction Kits, B-PER™

Description: B-PER® Bacterial Protein Extraction Reagents are designed to extract soluble protein from bacterial cells. The B-PER® Bacterial Protein Extraction Kit eliminates the need for harsh mechanical procedures, like sonication. The easy-to-use cell lysis solution uses mild non-ionic detergents to disrupt cells and solubilise proteins without denaturation. Enzyme supplements such as DNase I and Lysozyme allow for improved yield of large molecular weight proteins which are difficult to purify. B-PER® is also compatible with downstream protein purification methods such as GST purification and His-tagged immobilised metal affinity chromatography (IMAC).
Catalog Number: PIER78260
UOM: 1 * 250 mL
Supplier: Thermo Fisher Scientific
Product Image-786-182

Protein fractionation kit, nuclear and cytoplasmic extraction kit

Description: The Nuclear and Cytoplasmic Extraction kit is for the enrichment of cytoplasmic and nuclear fractions from cultured cells and tissues. The kit generates a cleaner separation of cytoplasmic proteins from nuclear proteins and is ideal for expression and transport studies.
Catalog Number: 786-182
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-786-231

FOCUS™ protein reduction-alkylation kit

Description: The FOCUS™ Reduction-Alkylation Kit offers a simple two-step method for reduction and alkylation of protein samples for 2D gel analysis.
Catalog Number: 786-231
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-786-230

FOCUS™ protein reductant kit

Description: FOCUS™ Protein Reductant Kit is a water soluble, odorless, non-toxic and stable TCEP [Tris (2-carboxyethyl) phosphine] reductant for protein reduction.
Catalog Number: 786-230
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-PIER89899

Ubiquitin enrichment kit, Pierce™

Description: The Pierce™ Ubiquitin enrichment kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples.
Catalog Number: PIER89899
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Image Unavailable-PIER84868

High pH reversed-phase kit, Pierce™

Description: Pierce™ High pH Reversed-Phase Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.
Catalog Number: PIER84868
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
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