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Protein expression systems make it very easy to sensitively detect various proteins used widely in bioanalytics and molecular biology. Fluorophores or certain vitamins are used to tag antibodies or other proteins, and these labeled molecules can then be purified or detected using other solutions such as streptavidin or monomeric avidin. These protein expression systems are optimized for the rapid synthesis of functionally active proteins.

Protein expression systems make it very easy to sensitively detect various proteins used widely in bioanalytics and molecular biology. Fluorophores or certain vitamins are used to tag antibodies or other proteins, and these labeled molecules can then be purified or detected using other solutions such as streptavidin or monomeric avidin. These protein expression systems are optimized for the rapid synthesis of functionally active proteins.


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TriLink® CleanCap® Cas9 Nickase mRNA (5 moU)

Supplier: TRILINK BIOTECHNOLOGIES
Description: <p>Cas9 Nickase mRNA expresses a version of the <em>Streptococcus pyogenes</em> SF370 Cas9 protein (CRISPR Associated Protein 9) that contains a D10A amino acid substitution. This mRNA also contains a C-terminal nuclear localisation signal followed by a HA tag.</p>

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TriLink® CleanCap® OVA mRNA (5 moU)

Supplier: TRILINK BIOTECHNOLOGIES
Description: Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunisation and biochemical studies and is an established model allergen for airway hyper-responsiveness.

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TriLink® CleanCap® Beta gal mRNA (5moU)

Supplier: TRILINK BIOTECHNOLOGIES
Description: β-galactosidase (β-gal), a protein product of the bacterial LacZ gene, catalyzes the conversion of β-galactosides into monosaccharides. β-gal is a common marker gene used to assess transfection efficiency.

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TriLink® CleanCap® Beta gal mRNA

Supplier: TRILINK BIOTECHNOLOGIES
Description: β-galactosidase (β-gal), a protein product of the bacterial LacZ gene, catalyzes the conversion of β-galactosides into monosaccharides. β-gal is a common marker gene used to assess transfection efficiency.

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Affinity protein expression and purification systems

Catalog Number: (AGLS214303)
Supplier: AGILENT
Description: The affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification.
UOM: 1 * 10 mL
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Freund's adjuvants

Supplier: Rockland Immunochemicals
Description: Freund's complete adjuvant

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TriLink® CleanCap® FLuc mRNA

Supplier: TRILINK BIOTECHNOLOGIES
Description: The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

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TriLink® CleanCap® M6 EPO mRNA (N1MePsU)

Supplier: TRILINK BIOTECHNOLOGIES
Description: <p>EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko et al. showed that transfection of EPO mRNA <em>in vivo</em> resulted in significant increases of both reticulocyte counts and hematocrits.</p>

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ALiCE for Research

Supplier: LENIOBIO
Description: From ALiCE® for Research to ALiCE® for Scale-Up Protein Expression, we offer cell-free Protein Expression products with radical results for every production setup.

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Squalene emulsion, SqualVax

Supplier: OZ BIOSCIENCES
Description: SqualVax is an oil-in-water emulsion made of squalene droplets in a continuous aqueous phase. It is an important advantage over alternative oils that have been used in emulsion adjuvants, like Freund’s adjuvant that contains mineral oil (paraffin oil) and has long term persistence in organisms.

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ProLite™ His-Tag Protein Gel Staining Kit

Catalog Number: (AATB18010)
Supplier: AAT BIOQUEST
Description: This kit requires very little hands-on time, allowing for rapid protein expression screening in a variety of gel types using an imaging equipment with a standard FITC filter.
UOM: 1 * 10 items
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Freund's incomplete adjuvant, Imject™

Catalog Number: (PIER77145)
Supplier: Thermo Fisher Scientific
Description: Imject™ Freund's Incomplete Adjuvant (FIA) is a popular non specific stimulator of the immune response for use in antibody production immunisation protocols.
UOM: 1 * 1 SET
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Alum adjuvant, AlumVax hydroxide

Supplier: OZ BIOSCIENCES
Description: AlumVax hydroxide is a wet gel (colloidal) of aluminium hydroxide 2%. It is the most common adjuvant used in approved prophylactic vaccines because of its excellent safety profile and ability to enhance the protective humoral immune response.

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Alum adjuvant, AlumVax phosphate

Supplier: OZ BIOSCIENCES
Description: AlumVax phosphate is a wet gel (colloidal) of aluminium phosphate 2%. Aluminium salts are the most common adjuvants used in approved prophylactic vaccines because of their excellent safety profile and ability to enhance the protective humoral immune response.

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Cell-free protein expression kits, 1-step human coupled IVT kit - DNA

Supplier: Thermo Fisher Scientific
Description: The 1-Step Human Coupled IVT Kit – DNA is a mammalian<i> in vitro</i> translation (IVT) system based on HeLa cell lysates. The kit contains all of the cellular components required for protein synthesis, including ribosomes, initiation factors, elongation factors and tRNA. When supplemented with the included proprietary accessory proteins, reaction mix and a DNA template cloned into the Thermo Scientific pT7CFE1-based vector, this system can synthesise protein for up to 6 hours. The kit includes a pT7CFE-CHis vector for ease of use in downstream applications.

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Cell-free protein expression kits, 1-step human high yield mini IVT kit

Supplier: Thermo Fisher Scientific
Description: The Thermo Scientific 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
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Product(s) marked with this symbol are discontinued - sold till end of stock. Alternatives may be available by searching with the VWR Catalogue Number listed above. If you need further assistance, please call VWR Customer Service on 0800 22 33 44.
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