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Product Image-EDVO243

Experimentation kits, ion exchange chromatography

Description: Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of two chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.
Catalog Number: EDVO243
UOM: 1 * 1 items
Supplier: EDVOTEK

SDS


Product Image-BE-415

Experimentation kits, ion exchange chromatography

Description: Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.
Catalog Number: BE-415
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Product Image-EDVO300

Experimentation kits, blue/white cloning of a DNA fragment and assay of ß-galactosidase

Description: When DNA is subcloned in the pUC polylinker region, β-galactosidase production is interrupted, resulting in the inability of cells to hydrolyse X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA ligase. Following the ligation to synthesis the recombinant plasmid, competent <i>E. coli</i> cells are transformed and the number of recombinant antibiotic-resistant white and blue colonies are counted. β-galactosidase activity is assayed from blue and white bacterial cells.
Catalog Number: EDVO300
UOM: 1 * 1 KIT
Supplier: EDVOTEK
Product Image-BE-501

Experimentation kits, antigen-antibody interactions

Description: This lab activity is designed to study highly specific lock-key matching properties of antigen-antibody and how this highly specific interaction can be exploited as a tool for research and analysis. This study involves the use of an immunodiffusion technique in which antigen and antibody are allowed to diffuse in a solid agarose medium. When antigen and antibody meet, antigen-antibody complex is formed, which leads to precipitation. Antigen-antibody precipitate is formed in the zone where the concentration of the two matching pairs reaches an optimal known as the zone of equivalence, which results in formation of a visible opaque precipitate region in agarose medium. Those regions of precipitation can be used for determination of concentration or titer of both antigen and antibody. This Antigen-Antibody Interaction kit is a hands-on study of both Ouchterlony Double Diffusion and Radial Immunodiffusion techniques. This kit also provides additional guidance materials for teaching other types of antigen-antibody interactions concepts such as immunoelectrophoresis and immunoprecipitation.
Catalog Number: BE-501
UOM: 1 * 1 KIT
Supplier: G-Biosciences
Image Unavailable-VWRS5640.0010

Package material, general

Description: Package material, general
Catalog Number: VWRS5640.0010
UOM: 1 * 10 items
Supplier: VWR Collection
Product Image-BINH1003101

Vane wheel for heat/air flow

Description: Vane wheel device is used for demonstrating air and heat flows generated by a burning candle, water vapour or other heat sources. Made of Aluminium and mountable on a long needle.
Catalog Number: BINH1003101
UOM: 1 * 1 items
Supplier: 3B Scientific
Product Image-EDVO271

Experimentation kits, simulation of HIV detection by ELISA

Description: An HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient's blood and adding them to a microtitre plate coated with HIV antigen. If HIV antibodies are present, they will bind to the antigens on the plate. In this experiment, students perform an ELISA text by coating microtitre plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.
Catalog Number: EDVO271
UOM: 1 * 1 items
Supplier: EDVOTEK
Product Image-EDVO110

Experimentation kits, molecular weight determination of proteins (agarose-based)

Description: This experiment introduces a simple method to determine protein subunit molecular weights using horizontal electrophoresis. As the protein standards and 'unknowns' are prestained, the separation of proteins can be observed during electrophoresis. Included in the experiment is protein-grade agarose, which provides an alternative to the use of polyacrylamide gels.
Catalog Number: EDVO110
UOM: 1 * 1 KIT
Supplier: EDVOTEK
Product Image-EDVO315

Kits: in search of the sickle cell gene by southern blot, Ready-to-Load™

Description: Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane the method allows the precise analysis and identification of DNA bands on a gel. Using this kit, students use Southern blotting to find a point mutation in the haemoglobin gene indicating Sickle Cell Anaemia.
Catalog Number: EDVO315
UOM: 1 * 1 items
Supplier: EDVOTEK

SDS


Product Image-EDVO212

Experimentation kits, cleavage of Lambda DNA with Eco RI endonuclease: intro to restriction enzymes

Description: The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for <i>Eco</i> RI (5 distinct sites, 2 are very close in size). In this experiment, Lambda DNA is digested by the <i>Eco</i> RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.
Catalog Number: EDVO212
UOM: 1 * 1 KIT
Supplier: EDVOTEK
Product Image-BINH1014525

Tube holder S

Description: Tube holder to support all S series electron tubes for easy and safe operation. The five pin sockets for the tube are concealed inside the tube holder. A cathode protection switch is integrated into the tube holder, to protect the heated cathode from excessive voltage. The base plate has a slot for attaching the Helmholtz pair of coils S.
Catalog Number: BINH1014525
UOM: 1 * 1 items
Supplier: 3B Scientific
Product Image-EDVO123

Nucleic acid testing for COVID-19

Description: Hands-on simulation of molecular testing for COVID-19.
Catalog Number: EDVO123
UOM: 1 * 1 KIT
Supplier: EDVOTEK
Product Image-EDVO953

Experimentation kits, Water Quality Testing III: Multiplex PCR testing of water contaminants

Description: Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required. One such test uses PCR in multiplex format. In this experiment, students test for the presence of three separate, classroom-safe organisms in a water sample using a single PCR reaction.
Catalog Number: EDVO953
UOM: 1 * 1 items
Supplier: EDVOTEK
Product Image-EDVO957

Experimentation kits, DNA damage and repair

Description: According to the World Health Organisation, between two and three million cases of skin cancer occur globally every year. Many of these cases are caused by preventable damage to DNA by UV light. In this experiment, students expose plasmid DNA to shortwave UV light to simulate the effect of sunbathing. The DNA is then analysed by agarose gel electrophoresis to observe the damage.
Catalog Number: EDVO957
UOM: 1 * 1 KIT
Supplier: EDVOTEK
Product Image-EDVO135

Using CRISPR to treat cystic fibrosis

Description: Hands-on simulation of CRISPR-Cas9 to target and treat Cystic Fibrosis.
Catalog Number: EDVO135
UOM: 1 * 1 KIT
Supplier: EDVOTEK
Product Image-EDVO990

Experimentation kits, morphology of cancer cells

Description: When normal cells are grown in culture they stop growing when they become overcrowded (contact inhibition). Cancer cells in culture grow in an uncontrolled way because they have lost this property. This helps tumours to form in the body. In addition, many different cell types can be present in a single tumour. This experiment allows students to see the differences between normal and cancer cells in both their growth and cell types.
Catalog Number: EDVO990
UOM: 1 * 1 items
Supplier: EDVOTEK
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