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Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.

Efficient cell lysis and maximum protein extraction yields are vital to high-quality recombinant protein purification. The protease inhibitor cocktails are for lysing mammalian cells, buffers that solubilize plasma and intracellular membranes, break weak intermolecular bonds, and solubilize most proteins. Making procedures easier to perform, these protein extraction reagents can also be used to isolate proteins from both the cytoplasmic and nuclear fractions of cultured cells.


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Product Image-PIER88216

Micrococcal nuclease solution (≥100 U/µl)

Description: This Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.
Catalog Number: PIER88216
UOM: 1 * 1 items
Supplier: Thermo Fisher Scientific
Product Image-786-790

Immobilised Papain

Description: A cysteine protease enzyme (EC 3.4.22.2) immobilised on 4% agarose, cleaves Immunoglobulin G (IgG) antibody molecules in the hinge region, generating three ~50 kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonisation, and lysis.
Catalog Number: 786-790
UOM: 1 * 1 items
Supplier: G-Biosciences
Product Image-786-791

Immobilised Pepsin

Description: A proteolytic enzyme immobilised on 4% agarose that is routinely used for the generation of F(ab’)₂ fragments from Immunoglobulin G (IgG). The pepsin has the ability to cleave the heavy chains near the hinge region. The immobilised Pepsin offers the distinct advantage of eliminating enzyme contamination of the F(ab)₂ fragments.
Catalog Number: 786-791
UOM: 1 * 1 items
Supplier: G-Biosciences
Product Image-PIER20308

SDS precipitation kit, SDS-Out™

Description: SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0,1 to 10 ml) protein samples.
Catalog Number: PIER20308
UOM: 1 * 1 KIT
Supplier: Thermo Fisher Scientific
Product Image-PIER85117

Detergent solutions, Surfact-Amps™

Description: Surfact-Amps™ detergents are ready-to-use, diluted solutions of purified detergent that can be used in routine and high-demand protein research methods and molecular biology techniques. These detergents are useful as a component of cell lysis buffers or other solutions intended to extract and solubilise proteins, improve chromatography, prepare yeast spheroplasts, and reduce background in ELISA or Western blot methods.
Catalog Number: PIER85117
UOM: 1 * 50 mL
Supplier: Thermo Fisher Scientific
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Endoproteinase Glu-C, excision grade, Staphylococcus aureus

Description: Native endoproteinase Glu-C from <i>Staphylococcus aureus</i>. Serine protease that specifically hydrolyses peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
Catalog Number: 324713-50
UOM: 1 * 50 µG
Supplier: Merck Millipore (Calbiochem‎)
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PopCulture® reagent

Description: PopCulture® Reagent is a detergent-based concentrate that can be added directly to cultures of <i>E. coli</i> to effectively extract proteins without the need for cell harvest. Recombinant proteins can be directly screened in the crude extract, or purified by adding an affinity matrix, washing the matrix-target protein complex to remove spent culture medium and cellular contaminants, and eluting the purified protein from the matrix. The PopCulture protein purification procedure is ideally suited to high-throughput robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience.
Catalog Number: 71092-4
UOM: 1 * 75 mL
Supplier: Merck Millipore (Novagen)
Product Image-PIER90305

Trypsin protease, MS grade

Description: Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.
Catalog Number: PIER90305
UOM: 1 * 100 µG
Supplier: Thermo Fisher Scientific

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Product Image-71009-4

Protein extraction reagents, CytoBuster™

Description: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.
Catalog Number: 71009-4
UOM: 1 * 250 mL
Supplier: Merck Millipore (Novagen)
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Master mix, BugBuster®

Description: BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.
Catalog Number: 71456-4
UOM: 1 * 500 mL
Supplier: Merck Millipore (Novagen)
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Endoproteinase Asp-N, excision grade, Pseudomonas fragi

Description: Native endoproteinase Asp-N from <i>Pseudomonas fragi.</i> Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
Catalog Number: 324708-2
UOM: 1 * 2 µG
Supplier: Merck Millipore (Calbiochem‎)
Product Image-786-680

Washing solution, Protein-OUT™

Description: Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.
Catalog Number: 786-680
UOM: 1 * 50 mL
Supplier: G-Biosciences
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Uniform latex particles for immobilisation of biomolecules, PL-Latex

Description: Uniform latex particles are used in a wide range of applications that exploit their physico-chemical properties in sophisticated immunoassay formats, both in medicine and veterinary science research.
Catalog Number: VARIPL6202-6102
UOM: 1 * 1 items
Supplier: VARIAN
Product Image-REST25850

QuEChERS extraction salts, Q-sep®

Description: QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.
Catalog Number: REST25850
UOM: 1 * 50 items
Supplier: Restek
Image Unavailable-CEMATMS001-4L

TamiSolve® NxG-PS Solvents for Peptide Synthesis

Description: Modern peptide synthesis agents can dramatically reduce waste generation, but challenges remain regarding the health and environmental impacts of commonly used solvents.
Catalog Number: CEMATMS001-4L
UOM: 1 * 4 L
Supplier: CEM

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Surfactant, RapiGest™ SF

Description: RapiGest™ SF is an anionic surfactant for peptide digestion. Supplied as five 1 mg vials for 1 to 50 digestions. The digestion speed and more peptide recovery is enhanced by using trypsin or other enzymes.
Catalog Number: WATE186001861
UOM: 1 * 5 items
Supplier: WATERS
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