You Searched For: EUPROMOCHEM00002

An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.

An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.


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Description: The Biosensis Mouse and Rat proNGF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rodent full-length proNGF protein in less than 4 hours in cell culture supernatants, cell lysates and tissue homogenates only if used as directed. This ELISA Kit has also successfully been used on mouse urine samples (Ryu JC et al., 2018). Please refer to the kit protocol for specific use instructions for each substrate application.

This ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli and consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A proNGF positive control (QC sample) is provided to assure consistent assay performance. This ELISA kit detects rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates and rat brain tissue homogenate. In the absence of a true rat proNGF standard, results may be expressed as 'mouse proNGF equivalents'. This ELISA kit shows only 20% reactivity with the human form of proNGF and is therefore not suitable to quantify human proNGF. No cross-reactivity was observed with mature mouse NGF and full-length proBDNF when tested in assay buffer.

The antibodies used in this ELISA kit bind epitopes within the pro-domain (capture) and mature domain (detection) of the protein, thus this ELISA assay does not detect the pro-domain peptide. This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required.
Catalog Number: BSENBEK-2236-1P
UOM: 1 * 96 Tests
Supplier: Biosensis


Description: The Biosensis NGFR/p75ECD Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of mouse p75 neurotrophin receptor in less than 4 hours in cell culture supernatants and urine only if used as directed.
Catalog Number: BSENBEK-2220-1P
UOM: 1 * 96 Tests
Supplier: Biosensis


Description: For the measurement of NET-derived neutrophil elastase.
Catalog Number: CAYM601010-1
UOM: 1 * 1 items
Supplier: Cayman Chemical


Description: The EarlyTox™ Cardiotoxicity Kit provides a fast, simple and reliable fluorescence based method for identifying cardiotoxic compounds in a biorelevant assay using stem-cell derived cardiomyocytes.
Catalog Number: MLDVR8211
UOM: 1 * 1 KIT
Supplier: Molecular Devices


Description: High quality RT-PCR kits for detection of various infectious diseases.
Catalog Number: GSDCPCOV6111
UOM: 1 * 1 KIT
Supplier: GOLD STANDARD DIAGNOSTICS


Description: A colorimetric-based method for detecting L-lactate in culture medium.
Catalog Number: CAYM600450-1
UOM: 1 * 1 items
Supplier: Cayman Chemical


Description: Superoxide Dismutase Assay (SOD) kit is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates and cell lysates.
Catalog Number: CAYM706002-96
UOM: 1 * 96 Tests
Supplier: Cayman Chemical


Description: Conjugate stabilisers are used with horseradish peroxidase (HRP) and calf intestinal alkaline phosphatase conjugated primary or secondary antibodies or other proteins or compounds in solution. The components of the stabiliser (proprietary) are unique and stabilise both the antibody and enzyme portions of the conjugate.
Catalog Number: ROCKMB-061-0100
UOM: 1 * 100 mL
Supplier: Rockland Immunochemicals


Description: Cellular assay, Cell-based assay calcein AM
Catalog Number: CAYM400146-1
UOM: 1 * 1 items
Supplier: Cayman Chemical


Description: The Biosensis proBDNF (Brain-derived neurotrophic factor, BDNF, Abrineurin) RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of proBDNF in less than 4 hours in cell culture supernatants, human serum and EDTA-plasma only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human blood samples.
Catalog Number: BSENBEK-2237-1P
UOM: 1 * 1 KIT
Supplier: Biosensis


Description: The set contains five reagents that induce apoptosis through different mechanisms.
Catalog Number: ENZOALX850235KI01
UOM: 1 * 1 SET
Supplier: ENZO LIFE SCIENCES


Description: This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy.
Catalog Number: ENZOENZ51001200
UOM: 1 * 1 KIT
Supplier: ENZO LIFE SCIENCES


Description: For the detection of apoptotic cells by flow cytometry.
Catalog Number: ENZOALX850253KI02
UOM: 1 * 100 Tests
Supplier: ENZO LIFE SCIENCES


Description: Ultrasensitive PGE2 ELISA kit for inflammation and eicosanoid research.
Catalog Number: ENZOADI930001
UOM: 1 * 1 KIT
Supplier: ENZO LIFE SCIENCES


Description: For use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins.
Catalog Number: ENZOENZ51005500
UOM: 1 * 500 Tests
Supplier: ENZO LIFE SCIENCES


Description: The LEADING LIGHT® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format.
Catalog Number: ENZOENZ610010001
UOM: 1 * 1 KIT
Supplier: ENZO LIFE SCIENCES


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