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Product Image-E506-100ML

Calcium chloride 1 mol/l in aqueous solution, Biotechnology Grade

Supplier: VWR Chemicals
Description: Reagent for the preparation of competent cells for transformation.

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Product Image-E525-500ML

Magnesium chloride 1 mol/l (2 N) in aqueous solution for biotechnology, sterile

Supplier: VWR Chemicals
Description: A sterile reagent for the preparation of competent cells for transformation.

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Automated plate washing for ELISA, cells and beads

Improve lab efficiency & optimize results with fast and efficient plate washers. Compare and find the optimal plate washer for your applications.

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TECAN microplate washers

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Lithium iodide hydrate ≥99.995% (metals basis), Puratronic®

Supplier: Thermo Scientific
Description: Lithium iodide hydrate is used as a reagent for ester cleavage and decarboxylation and C-C bond forming reactions. It is also used as source of nucleophilic iodide, as a mild lewis acid, epoxide opening and as an additive for organometallic-mediated transformations.

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Product Image-K1232

PCR cloning kit, CloneJET

Supplier: Thermo Fisher Scientific
Description: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Product Image-AGLS200519

Mutagenesis kits QuikChange

Supplier: AGILENT
Description: The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 to 14 kb) or otherwise difficult-to mutagenise plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.

Image Unavailable-BNUM1065-50

Anti-ABO Mouse Monoclonal Antibody (Purified, BSA-free) [clone: HEB-29]

Catalog Number: (BNUM1065-50)
Supplier: Biotium
Description: The antibody HEB-29 reacts with human blood group B. The specificity of the antibody HEB-29 was confirmed by comparison of specificity and reactivity to standard reagent using >5.000 samples of blood. MAb HEB-29 shows specific staining of erythrocytes and vascular epithelium of blood group B controls and no staining in group A controls. It is applicable for tissue staining in tumor patients with blood groups B and AB. Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.
UOM: 1 * 50 µl
Image Unavailable-BNUB1065-500

Anti-ABO Mouse Monoclonal Antibody (Purified) [clone: HEB-29]

Supplier: Biotium
Description: The antibody HEB-29 reacts with human blood group B. The specificity of the antibody HEB-29 was confirmed by comparison of specificity and reactivity to standard reagent using >5.000 samples of blood. MAb HEB-29 shows specific staining of erythrocytes and vascular epithelium of blood group B controls and no staining in group A controls. It is applicable for tissue staining in tumor patients with blood groups B and AB. Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

Image Unavailable-J60028.AK

Transformation reagent, TB solution-II

Catalog Number: (J60028.AK)
Supplier: Thermo Scientific
Description: For transformation of competent cells.
UOM: 1 * 250 mL

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Image Unavailable-ICNA112100200

Transformation kit, EZ-Yeast™, MP Biomedicals

Catalog Number: (ICNA112100200)
Supplier: MP Biomedicals
Description: The EZ-Yeast™ Transformation Kit is designed for high throughput transformation with efficiencies up to 10³ transformants per μg plasmid DNA. It is ideal for cases where a large number of transformations must be performed, but only a few transformants are needed. Perfect for a quick analysis of potentially positive two-hybrid colonies, it does not require fully competent cells prior to transformation.
UOM: 1 * 200 Tests
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Yeast transformation kit, Alkali-Cation™, MP Biomedicals

Catalog Number: (ICNA112200200)
Supplier: MP Biomedicals
Description: Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transformation and has been successfully used to transform linear DNA into <i>Pichia pastoris</i> for protein expression studies.
UOM: 1 * 250 Tests
Product Image-GZ-1

Yeast transformation kit, Fast Yeast Transformation™

Catalog Number: (GZ-1)
Supplier: G-Biosciences
Description: Fast Yeast Transformation™ is a rapid single step yeast transformation kit that takes less than 10 minutes to prepare competent yeast cells. The competent yeast cells can be used immediately or frozen for later use. This method is suitable for both circular and linear plasmid transformations.
UOM: 1 * 1 KIT
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Electrofusion buffer, BTXpress Cytofusion® Medium C

Catalog Number: (731-0348)
Supplier: BTX
Description: BTXpress Cytofusion® Medium C is an advanced electrofusion buffer designed especially for hybridoma production. The low conductivity buffer is specially formulated to minimise cell turbulence during cell alignment and heating during electrofusion, for robust cell fusion efficiency and high cell viability. BTXpress Cytofusion® Medium C is sterile filtered from the highest quality non animal, medical grade reagents.
UOM: 1 * 500 mL
Product Image-700-4813

Flatpack chambers

Catalog Number: (700-4813)
Supplier: BTX
Description: Flatpack chambers are primarily used for prokaryotic applications (bacterial or yeast transformations), however they are also often used for high efficiency stem cell transfection.
UOM: 1 * 50 items
Product Image-BTXI47-0003

Electroporation buffer, BTXpress Cytoporation® Medium T and T4

Supplier: BTX
Description: BTXpress Cytoporation® Media T/T4 is an advanced electroporation buffer designed for use with the BTX AgilePulse MAX™ large volume electroporation system for <i>in vitro</i> delivery of DNA, RNA, oligonucleotides, and siRNA. The low conductivity buffer is specially formulated to minimise heating of solution during large volume electroporation for maximum transfection efficiency and high cell viability. BTXpress Cytoporation® Media T and T4 are sterile filtered from the highest quality non animal, medical grade reagents. Buffer can be directly diluted in complete growth media for post electroporation cell culture.

Product Image-SARTFT-3-343-047

Filter boards

Supplier: Sartorius
Description: Filter boards are used for filtration of cooking oils, transformer oils and as base paper for impregnation with certain reagents.

Product Image-732-1285

Electroporation solution kits, BTXpress™

Supplier: BTX
Description: The BTXpress™ is a single buffer solution, developed to quickly and efficiently deliver genes into mammalian cells that were previously considered hard to transfect by chemical and other non viral methods. Transfection using this high performance electroporation solution is equally effective in delivering DNA as well as siRNA into mammalian cells.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us on 0800 22 33 44
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
Additional Documentation may be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organisation. Please contact your purchasing department for more information.
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