5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (BCIP (p-toluidine salt), X-Phosphate) substrate ≥99%

Supplier: Thermo Fisher Scientific
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Synonyms: BCIPP-toluidine 5-bromo-4-chloro-1H-indol-3-yl phosphate

PIER34040EA 381 GBP
PIER34040
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (BCIP (p-toluidine salt), X-Phosphate) substrate ≥99%
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (BCIP (p-toluidine salt), X-Phosphate)

Thermo Scientific Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.


  • AP substrate—for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples
  • Chromogenic—no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph
  • Package options—choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor
  • Product is shipped at ambient temperature.


The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes


Highlights of Pierce NBT/BCIP 1-Step Solution (Part No. 34042):
Best for immunoblotting applications
Ready-to-use single component
Sensitive with low background and minimial assay-to-assay variability


Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity).


Caution: For Research Use Only. Not for use in diagnostic procedures.

Formula: C₁₅H₁₅BrClN₂O₄P
MW: 433.6214 g/mol
Storage Temperature: Freezer
MDL Number: MFCD00040636
CAS Number: 6578-06-9
EINECS: 229-506-1

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Specification Test Results

VISUAL White to off-white crystalline solid, free of foreign matter.
WATER CONTENT (KF) ≤ 2%
MOLAR ABSORPTIVITY ≥ 5700 at ~292 nm in 10mM Diethanolamine, pH 10.3 at 25 °C
PURITY (HPLC) ≥ 99%
INORGANIC PHOSPHATE ≤ 0.1%

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