ABTS (Diammonium 2,2'-azinobis[3-ethyl-2,3-dihydrobenzothiazole-6-sulphonate]) substrate, powder for ELISA
Supplier: SOUTHERN BIOTECHN.Total Ratings: 0
Avg. Ratings: 0.0 out of 5
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Synonyms:
2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt
, Ammonium 2,2'-(hydrazine-1,2-diylidene)bis(3-ethyl-2,3-dihydrobenzo[d]thiazole-6-sulphonate)
, ABTS
ABTS Substrate Powder for ELISA Produces a soluble, blue-green end product upon reaction with horseradish peroxidase (HRP) that is measurable between 405 nm and 410 nm .
Product Use
Substrate buffer - To 50 mL of distilled water, add 525 mg citric acid. Adjust pH to 4.0 with 3 N NaOH. Prepare before each use.
Substrate solution - Dissolve 15 mg ABTS powder in 1.0 mL of distilled water and store at 2-8C protected from light. Just prior to use, add 200 µL ABTS solution and 10 µL of 30% H2O2 to 10 mL substrate buffer.
Reaction may be stopped with 1% sodium dodecyl sulfate (SDS)
Formula:
C₁₈H₂₄N₆O₆S₄ MW: 548.68 g/mol Storage Temperature: Refrigerator |
MDL Number:
MFCD00010404 CAS Number: 30931-67-0 EINECS: 250-396-6 |
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