3,3'-Diaminobenzidine tetrahydrochloride hydrate, ISOPAC®, Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: DAB tetrahydrochloride hydrateDAB HCl hydrate3,3',4,4'-Biphenyltetramine tetrahydrochloride hydrate

M-Clarity™ quality level = MQ100

D9015-100MGEA 70.7 GBP
D9015-100MG
3,3'-Diaminobenzidine tetrahydrochloride hydrate, ISOPAC®, Sigma-Aldrich®
3,3'-Diaminobenzidine tetrahydrochloride hydrate

3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridisation. DAB (3,3′-Diaminobenzidine) is utilised in many applications for the visualisation of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidised DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.


Packaging: Weight approximate. Packaged in a 100 ml serum bottle with silicon/PTFE septum and aluminum tear seal.


Caution: Injecting a compatible solvent permits preparation of any desired strength solution without exposure.

Formula: C₁₂H₁₄N₄·4HCl·H₂O
MW: 360.11 g/mol (anhy)
Melting Pt: 280 °C
Storage Temperature: Room Temperature
MDL Number: MFCD08273058
CAS Number: 868272-85-9
EINECS: 231-018-9

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Specification Test Results

Appearance (Colour); Off-White to Faint Red to Beige Conforms to Requirements
Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Purity (TLC) ≥96%
Solubility (Turbidity), c = 50 mg/ml; Water Clear to Slightly Hazy
Solubility (Colour); Faint Pink to Amber Conforms to Requirements
Water (by Karl Fischer) 4.0 - 10.0%
Recommended Retest Period 2 Years

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