3,3'-Diaminobenzidine tetrahydrochloride hydrate, ISOPAC®, Sigma-Aldrich®
Supplier: SIGMA-ALDRICH MICROSCOPYTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
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Danger
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Synonyms:
DAB tetrahydrochloride hydrate
, DAB HCl hydrate
, 3,3',4,4'-Biphenyltetramine tetrahydrochloride hydrate
M-Clarity™ quality level = MQ100
3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridisation. DAB (3,3′-Diaminobenzidine) is utilised in many applications for the visualisation of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidised DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.
Packaging: Weight approximate. Packaged in a 100 ml serum bottle with silicon/PTFE septum and aluminum tear seal.
Caution: Injecting a compatible solvent permits preparation of any desired strength solution without exposure.
Formula:
C₁₂H₁₄N₄·4HCl·H₂O MW: 360.11 g/mol (anhy) Melting Pt: 280 °C Storage Temperature: Room Temperature |
MDL Number:
MFCD08273058 CAS Number: 868272-85-9 EINECS: 231-018-9 |
Specification Test Results
Appearance (Colour); Off-White to Faint Red to Beige | Conforms to Requirements |
Appearance (Form) | Powder |
Infrared spectrum | Conforms to Structure |
Purity (TLC) | ≥96% |
Solubility (Turbidity), c = 50 mg/ml; Water | Clear to Slightly Hazy |
Solubility (Colour); Faint Pink to Amber | Conforms to Requirements |
Water (by Karl Fischer) | 4.0 - 10.0% |
Recommended Retest Period | 2 Years |
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