The supplement kit for microvascular endothelial cell growth medium, advanced is suitable for culturing Provitro human microvascular endothelial cells after adding the supplement kit components to 500 ml basal medium. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.
IL-4 also regulates immunoglobulin class switching to IgG1 and IgE, playing a crucial role in immune responses. Excessive IL-4 production by Th2 cells is associated with elevated IgE levels and allergies.
Storage: Samples remain stable for a duration of twelve months from the receipt date when stored within the temperature range of −20 to −80 °C. To ensure optimal storage conditions, maintain a sterile environment at these temperatures. For improved preservation, it is advisable to aliquot the protein. Avoid subjecting the samples to repeated freeze-thaw cycles to prevent degradation and maintain the integrity of the material.
Caution: Store the supplements at −20 °C. Do not use the supplements after expiry date. Avoid any thaw/freeze cycle.
