The Provitro skeletal muscle cell growth medium is a sterile liquid culture medium for culturing human skeletal muscle cells (HSKMC). The medium is delivered as a basal medium and is suitable for culturing Provitro HSKMC after adding the supplement kit components. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.
It is also used in cell culture, particularly in systems with a low serum content or completely serum-free culture systems .This Product is produced from the yeast Pichia pastoris.
Storage: Samples remain stable for a duration of twelve months from the receipt date when stored within the temperature range of −20 to −80 °C. To ensure optimal storage conditions, maintain a sterile environment at these temperatures. For improved preservation, it is advisable to aliquot the protein. Avoid subjecting the samples to repeated freeze-thaw cycles to prevent degradation and maintain the integrity of the material.
Caution: The supplemented skeletal muscle cell growth medium can be stored in the dark at 2 to 8 °C for up to 1 month. Do not heat the medium over 37 °C or use uncontrollable sources of heat (e.g. microwave appliances). If only a part of the medium is to be used, remove this amount from the bottle and heat it.
Do not freeze the medium. This can lead to high salt concentrations by freezing out pure water which will cause irreversible damage.
