The supplement kit designed for cultivating human smooth muscle cells (HSMC) provides an optimised formulation for promoting growth and maintaining the health of these cells. Upon adding the components of the supplement kit to a 500 ml basal medium, a specialised environment is created to support the culture of HSMCs. The formulation is specifically tailored for the initial seeding of 4000 cells per square centimeter, aiming to achieve confluence at approximately 90%. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. All components are subjected to rigorous biological assays. Each batch is specifically examined for the proliferating characteristics of human smooth muscle cells (HSMC). The cells cultured in the smooth muscle cell growth medium undergo thorough assessments, including examination of their morphology, adherence rate, colony-forming efficiency, and population doubling time.
It is also used in cell culture, particularly in systems with a low serum content or completely serum-free culture systems.
Storage: Samples remain stable for a duration of twelve months from the receipt date when stored within the temperature range of −20 to −80 °C. To ensure optimal storage conditions, maintain a sterile environment at these temperatures. For improved preservation, it is advisable to aliquot the protein. Avoid subjecting the samples to repeated freeze-thaw cycles to prevent degradation and maintain the integrity of the material.
Caution: Store the supplemented smooth muscle cells at a temperature of −20 °C. The supplements must not be used after the expiry date. Always adhere to these storage guidelines to maintain the effectiveness and integrity.
