4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP), Reagent Grade

Supplier: VWR Chemicals
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0364-25GEA 473 GBP
0364-25G
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP), Reagent Grade
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP)

Preferred substrate for high sensitivity detection of alkaline phosphatase in EIA assays. Read at 405 nm.


Soluble.Reaction Condition: 1 mg/ml in 10 mM Diethanolamine and 0.5 mM MgCl2, pH 9.5.

Formula: C₆H₄NNa₂O₆P·6H₂O
Melting Pt: 300 °C
Storage Temperature: Freezer
MDL Number: MFCD00066288
CAS Number: 333338-18-4
EINECS: 224-246-5

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Specification Test Results

Em (312 nm, 0.15M AMP Buffer, pH 10.25) >9800
Inorganic Phosphate (% by Weight) <1.0%
p-Nitrophenol <0.1%

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