4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP), Reagent Grade
Supplier: VWR Chemicals
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0364-25GEA
473
GBP
0364-25G
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP), Reagent Grade
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP)
Preferred substrate for high sensitivity detection of alkaline phosphatase in EIA assays. Read at 405 nm.
Soluble.Reaction Condition: 1 mg/ml in 10 mM Diethanolamine and 0.5 mM MgCl2, pH 9.5.
Formula:
C₆H₄NNa₂O₆P·6H₂O Melting Pt: 300 °C Storage Temperature: Freezer |
MDL Number:
MFCD00066288 CAS Number: 333338-18-4 EINECS: 224-246-5 |
Specification Test Results
Em (312 nm, 0.15M AMP Buffer, pH 10.25) | >9800 |
Inorganic Phosphate (% by Weight) | <1.0% |
p-Nitrophenol | <0.1% |
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