3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS) zwitterionic detergent, Pierce™

Supplier: Thermo Fisher Scientific
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Synonyms: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulphonateCHAPS

PIER28299EA 1850 GBP
PIER28299
3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS) zwitterionic detergent, Pierce™
3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS)

Thermo Scientific CHAPS is a zwitterionic detergent that is especially well suited for protecting the native state of proteins.


  • Versatile zwitterionic detergent
  • Provides mild but effective lysis of cultured mammalian cells
  • Nondenaturing and generally does not inactivate protein functions
  • Can be removed from solution by dialysis
  • Commonly used for isoelectric focusing (IEF) and 2D electrophoresis


CHAPS is sulfobetaine derivative of cholic acid


CHAPS has been successfully used to solubilize intrinsic membrane proteins and receptors and maintain the functional capability of the protein of interest. CHAPS has also been used in combination with nonionic detergents such as NP-40 for nondenaturing gel electrophoresis applications. CHAPS has largely replaced NP-40 in isoelectric focusing (IEF) applications, where it prevents streaking in certain pH ranges. The neutral charge of CHAPS and its disaggregating properties are believed to be responsible for the improvement.


Properties of CHAPS Detergent:
Chemical Name: 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate
Molecular Weight: 614.88g
Detergent Class: Zwitterionic
Aggregation Number: 10
Micelle Molecular Weight: 6149g
Critical Micelle Concentration (CMC): 8 to 10 mM (0.4920 to 0.6150%, w/v)
Cloud Point: ≥100˚C
Dialyzable: Yes


Specifications for CHAPS:
Visual: White free-flowing powder, free of foreign matter.
Solubility: A 10% (aq) solution is clear, colorless.
Identity: The I.R. scan must show only peaks characteristic for the structure and functional groups of the compound being tested.
Purity: (TLC) One major spot. A small, secondary spot is acceptable.


Caution: For Research Use Only. Not for use in diagnostic procedures.

Formula: C₃₂H₅₈N₂O₇S
MW: 614.89 g/mol
Melting Pt: 116 °C
Storage Temperature: Refrigerator
MDL Number: MFCD00081083
CAS Number: 75621-03-3
Merck Index: 12,02085

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