Eosin Y (yellowish) 75% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA-ALDRICH MICROSCOPY
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Synonyms: Tetrabromofluorescein disodium saltEosin Y disodium saltEosin 2252′,4′,5′,7′-Tetrabromofluorescein disodium saltSolvent Red 43Disodium 2-(2,4,5,7-tetrabromo-6-oxido-3-oxoxanthen-9-yl)benzoateDisodium 2',4',5',7'-tetrabromo-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-bis(olate)Field's Stain B2,4,5,7-Tetrabromofluorescein disodium salt

M-Clarity™ quality level = MQ100

230251-25GEA 156 GBP
230251-25G
Eosin Y (yellowish) 75% (by HPLC), Sigma-Aldrich®
Eosin Y (yellowish)

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.


Eosin Y has been used as a reagent in the preparation of Nigrosin-Eosin (NE) stain and is used in hematoxylin and eosin (H&E) staining technique to visualise alginate.

Formula: C₂₀H₆Br₄Na₂O₅
MW: 691.86 g/mol
Melting Pt: 295…296 °C
Storage Temperature: Ambient
MDL Number: MFCD00005040
CAS Number: 17372-87-1
EINECS: 241-409-6
Merck Index: 13,03634
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Specification Test Results

Appearance (Colour) Orange to Very Dark Orange
Appearance (Form) Powder
Infrared spectrum Conforms to Structure
Wavelength 521 - 527 nm
Extinction Coefficient ≥90000
Wavelength 304 - 310 nm
Extinction Coefficient ≥14000
Wavelength 255 - 261 nm
Extinction Coefficient
In Methanol
≥30000
Purity (HPLC) ≥75.0%

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