Fluo-3, pentaammonium salt ≥95% (by HPLC), Ultrapure
Supplier: ENZO LIFE SCIENCES AGTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
Synonyms:
1-[2-Amino-5-(2,7-dichloro-6-hydroxy-3-oxo-3H-xanthen-9-yl)phenoxy]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid .5NH3
, Fluo-3
Calcium dye
Fluo-3 is widely used in flow cytometry, confocal laserscanning microscopy and fluorescence spectroscopy. The probe has proved particularly valuable for determining GPCR function via cell-based high-throughput screening. Fluo-3 is a membrane impermeable probe and can be loaded in cells by microinjection, electroporation or scrape loading. The acetomethoxy (AM) version is necessary for passive cell loading. Once inside the cell, Fluo-3 becomes fluorescent, in the presence of free Ca (II). Fluo-3 is a long wavelength calcium probe (488 nm excitable). The longer wavelength of absorption is convenient for minimizing photodamage in cells. Wavelength Maxima: Excitation 506 nm, Emission 526 nm
MW:
854.69 g/mol Storage Temperature: Freezer |
CAS Number:
339221-91-9 |
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